Universitiy, Science, Research

Besides multiphase material handling technology in offshore area multiphase blood flow is also one of the investigation objects at the Institute for Multiphase Processes, Leibniz Universitaet Hannover. Generation of micro- and nano-structured materials for Functional Tissue Engineering (FTE) demonstrates a new research focus. Further interdisciplinary foci are laid on the already established 2D- and 3D-laser based visualisation techniques for capturing flow and concentration fields in chemical boxes and their forthcoming application within biomedical technological bioreactors in FTE. X-ray tomographic visualisations of multiphase flows in bubble columns, fills, packing materials as well as explanted heart valve prostheses complete the interdisciplinary research papers (420 kV x-ray tomograph). In the field of Cryotechnics the institute deals with procedural development of low temperature long-term storage of biological cells and tissues using liquid nitrogen at -196 C° as well as room temperature storage through lyophilisation. Due to freeze-drying and electrospinning (in FOR polysialin) biomaterials for scaffolds for FTE is developed that induce with developed bioreactors also mechano-transduction for example on endothelial cells.

Cryopreservation of cells and 3d native and artificial tissue (TEP)

What we offer

Different cell types need special freezing and thawing conditions to minimise cell stress and to enhance cell survival. By means of systematic parameter optimisation optimal freezing protocols and suitable cryoprotective agents (CPA) can be established for different cell models.
Effective cryopreservation of native and engineered tissue poses a specific challenge for biomedical applications. The temperature distribution and CPA (cryoprotective agent) gradients are important in such three dimensional geometries.
The adaptation of cooling rates and concentrations of cryoprotectant besides the conformation of the thawing process are important to be defined for tissue samples with a three-dimensional conformation. Cryopreservation strategies for tissue- and tumor banking are developed so that functional studies after recultivation of cryopreserved cells are possible.

What we are looking for

We are looking for partners who are interested in optimizing their freezing protocols for their valuable cells and/or need a cryopreservation strategy for the long term storage of tissues for biobanking.
Additionally we would like to cooperate for the design and development of new controlled rate freezers.

Collaboration sought

  • Research & Development
  • Technical Co-operation


Ms Dr. Nicola Hofmann

Group Leader JRG Biothermodynamics, REBIRTH

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